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A Novel 10 Layer Flask For use in the SelecT Automated Cell Culture System

Poster   Mar 22, 2007

 
A Novel 10 Layer Flask For use in the SelecT Automated Cell Culture System
 
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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License

 
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Knockout of microRNAs Using the CRISPR-Cas9 System with Paired Synthetic crRNAs

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We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.

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Proteomics and Substrate Based MS Imaging of Xenobiotic Metabolising Enzymes in Ex Vivo Human Skin and a Human Living Skin Equivalent Model

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Untargeted proteomics analysis showed that human skin and a commercially available living skin equivalent model exhibit a similar distribution of xenobiotic metabolising enzymes. A new technique, substrate based mass spectrometry imaging (SB-MSI) was developed during this study.

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Development and Assessment of Non-Infected and Infected Skin Models Using MALDI-MSI

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Infections have detrimental effects on wound healing leading to chronic wound formation. The use of labskin,a living skin model, in combination with mass spectrometry imaging, provides a good model for assessing wound healing factors.

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