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A No-Wash No-Quench Assay for Phagocytosis Based on a Fluorogenic BioParticles Conjugate Made with the Novel pH Sensitive pHrodo Dye

Current protocols for measuring phagocytosis necessitate a trypan blue quenching step with several washes. These steps can introduce significant variability in the assay. To address this, we have developed a no-wash phagocytosis procedure using a novel pH sensitive dye.