A No-Wash No-Quench Assay for Phagocytosis Based on a Fluorogenic BioParticles Conjugate Made with the Novel pH Sensitive pHrodo Dye
Summary
Current protocols for measuring phagocytosis necessitate a trypan blue quenching step with several washes. These steps can introduce significant variability in the assay. To address this, we have developed a no-wash phagocytosis procedure using a novel pH sensitive dye.
Current protocols for measuring phagocytosis necessitate a trypan blue quenching step with several washes. These steps can introduce significant variability in the assay. To address this, we have developed a no-wash phagocytosis procedure using a novel pH sensitive dye.
