A systems analysis of the AHL Quorum Sensing system in Pseudomonas aeruginosa

Poster   Apr 29, 2009

 
A systems analysis of the AHL Quorum Sensing system in Pseudomonas aeruginosa
 
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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License

 
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Genomic Analysis of L5178Y tk+/- Cells and their Induced tk-/- Mutant Colonies

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The goal of this pilot study was to exploit NGS technology with a view to shedding new light on the genome of cells used in the MLA, as well as the changes induced as a result of the exposure to mutagens.

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Our results indicate that MS modifications are required for experiments with co-electroporation of Cas9 mRNA and synthetic gRNA, yet have no impact on editing efficiency when delivered with lipid-based transfection reagents.

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