A Walk-Up ADME Testing System for Medicinal Chemists and Pharmacologists
Poster Feb 08, 2008
Muhammad Alimuddina, Martin Peacock, Russell Dahl, Omar Jina, and Paul Oakley
VivADME was born with the fusion of a problem and a solution: recognition by a Medicinal Chemist that ADME data took too long to obtain, and, the realization that microfluidic tools could address the problem. The vivADME microfluidics solution means that ADME profiling can be performed in minutes with microgram-scale samples in a walk-up system with a very low cost per sample. Examples are given for log D determinations, Liver Microsomal Stability and Plasma Stability. Log D measured by the microfluidics method was compared to the traditional shaken-flask method on 28 drug-like and other molecules and gave a correlation R2 = 0.9751. Rapidly obtained VivADME results for Microsomal Stability and Plasma Stability correlate well with more labour-intensive ADME results obtained using standard protocols.
During early drug discovery, the study of metabolism plays an essential role in determining which drug candidates move forward into development and later stages. As an alternative to traditional Data Dependent Acquisition (DDA), the use of MSE/All Ions Fragmentation (AIF) has become common in metabolite identification workflows for the analysis of metabolic hot spots. Here we present a solution for analysis of MSE/AlF in metID studies.READ MORE