An Ultra-Sensitive Fluorimetric Assay for the Detection of Renin Activity

To facilitate high throughput screening of renin inhibitors, we have developed new renin assay kits utilizing FRET peptide substrates. The SensoLyteTM 520 Renin Assay Kit contains FRET peptide labeled with the quencher QXL™520 and the fluorophore 5-FAM. These newly developed assays were validated with several known renin inhibitors. Assay with QXL™ 520/5-FAM substrate was approximately 40 fold more sensitive than an existing assay which uses EDANS/DABCYL FRET substrate.