An Ultra-Sensitive Fluorimetric Assay for the Detection of Renin Activity
Poster Apr 24, 2007
To facilitate high throughput screening of renin inhibitors, we have developed new renin assay kits utilizing FRET peptide substrates. The SensoLyteTM 520 Renin Assay Kit contains FRET peptide labeled with the quencher QXL™520 and the fluorophore 5-FAM. These newly developed assays were validated with several known renin inhibitors. Assay with QXL™ 520/5-FAM substrate was approximately 40 fold more sensitive than an existing assay which uses EDANS/DABCYL FRET substrate.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE