Automation of a Novel Cell-Based ELISA for Cell Signaling Pathway Analysis
Poster Feb 16, 2012
Wendy Goodrich, Peter Banks, Ron Osmond, Antony Sheehan
In addition to offering simplicity, speed, and flexibility, the technology is a highly sensitive technique that lends itself to automation at multiple levels including seeding cells in microplates; cell media exchanges; serial dilution and transfer steps; assay reagent additions; microplate washing; and, signal detection. Assay performance studies were conducted using lysate kit controls to verify optimal automation performance for S/B, Z’, and intra- and inter-assay precision. A ‘one well one day’ cell based assay protocol was developed and used for pharmacology assessment for agonist/antagonist analysis of the PI-3/AKT/mTOR signaling pathway. Procedures demonstrated here are well suited for validating automation of model system protocol development.
Performance of the D5000 and High Sensitivity D5000 ScreenTape Assays for the 4200 TapeStation SystemPoster
Here, we focus on quantification, sizing, and sensitivity of both D5000 ScreenTape assays.READ MORE
CiPA Phase 2 Study: validation of an automated microelectrode array (MEA) assay of hiPSC-derived cardiomyocyte electrophysiology for cardiac safety evaluationPoster
These results support the use of hSC-CM and MEA technology for preclinical assessment of proarrhythmic risk within the proposed CiPA paradigm, and, more generally, demonstrate that automation of the CM-MEA assay can achieve high reliability and throughput for cardiac risk assessment in vitro.READ MORE