Calcium Detection Using the NOVOstar and Screen QuestT Fluo-8 NW Calcium Kit
Poster Oct 10, 2007
The Screen Quest™ Fluo-8 NW Calcium Assay Kits provide an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay was successfully performed on the NOVOstar. The NOVOstar from BMG LABTECH is the ideal platform to perform cell-based, fast kinetic assays such as intracellular calcium measurements, because it has a unique pipetting system that can add small amounts of agonist or antagonist (< 1.0 µl) to cells.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE