Fast PK and Semi Quantitative Analysis of Metabolites Using High Resolution MS
Poster Nov 19, 2010
Rohan A. Thakur and Mike Koleto
Fast PK quantitative studies are traditionally performed using a technique known as selected
reaction monitoring (SRM) on a triple quadrupole mass spectrometer. In the SRM mode, the ions
specific to the NCE are focused onto the detection system, while all other non-specific ions are filtered away. As a result, only the NCE is quantified and its PK estimates determined, but any metabolite information contained in the sample is lost. With the understanding of this limitation of the SRM technique is the acknowledgement that potentially efficacious NCEs may be prematurely eliminated due to poor PK characteristics, when in fact more complete feedback from the same Fast PK study could provide the support for the decision to make a simple tweak of the chemical structure to improve bioavailability. This is where Orbitrap technology (OT) has the potential to revolutionize Lead Optimization, by providing metabolite information during the Fast PK analysis.
CiPA Phase 2 Study: validation of an automated microelectrode array (MEA) assay of hiPSC-derived cardiomyocyte electrophysiology for cardiac safety evaluationPoster
These results support the use of hSC-CM and MEA technology for preclinical assessment of proarrhythmic risk within the proposed CiPA paradigm, and, more generally, demonstrate that automation of the CM-MEA assay can achieve high reliability and throughput for cardiac risk assessment in vitro.READ MORE
A luminescent solar concentrator-based photomicroreactor for energy efficient continuous-flow photocatalysisPoster
The Luminescent Solar ConcentratorPhotomicroreactor
(LSC-PM) is an innovative device
for solar-powered continuous-flow photochemistry.