Fragment Screening Using Capillary Electrophoresis
Poster May 14, 2010
Capillary electrophoresis, which was pioneered for HTS in the 1990s, has recently been adapted to enable the screening of fragments. The technique is explained and the screening of fragments against a chaperone protein Hsp90 is presented as an example.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE