Functional Screening of recombinant CHO-M1 cells using the PHERAstar from BMG LABTECH
Published: August 5, 2009
Functional screening of single cell clones was achieved using the HTRF® IP-One assay. The HTRF® assay from Cisbio proved to be a fast and reliable method to directly screen a large number of stable transfected single cell clones. Carbachol dose response curves with fresh cells from a growing culture and frozen instant cells showed similar results for EC50 and signal-to-blank values.