High Throughput Anchorage-Independent Cell Colony Growth Assay with the IsoCyte™-HTS Platform
Poster Feb 06, 2009
The advantages of anchorage-independent cell colony growth assays for drug development are well known. Monitoring proliferation of cells in colony form is more biologically relevant than as single cells and formation of 3-dimensional (3D) colonies in semi-solid media better represents
in vivo environments.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE