High Throughput Cell Cycle Analysis using Microplate Cytometry
Poster Feb 01, 2007
Tristan Cope and Wayne Bowen
The cell cycle is a target for many anti-cancer drugs and the ability to monitor the effects of such agents on the cell cycle is an important part of the drug development process. Standard methods measure changes in DNA content by staining the nuclei of fixed cells with propidium iodide. The cells are then sorted by flow cytometry into G1, S and G2/M populations according to fluorescent intensity. The main disadvantages of this technique are low throughput, use of large number of cells and an inability to analyse adherent cell lines in situ.
To improve screening efficiency, we have developed a cell cycle analysis method that uses an Acumen Explorer fluorescence microplate cytometer to measure the DNA content of propidium iodide stained fixed cells in microplates. We demonstrate that paclitaxel and vinblastine arrested CHO cells in the expected phase of the cell cycle.
Exploiting Polypharmacology in Precision Oncology: Identification of Differential Kinase Off-targets Among Clinical PARP InhibitorsPoster
Can we use computational methods to identify previously unknown off-targets of PARP inhibitors that can explain their observed differences?READ MORE
Bioluminescent Assay for GTPases Allows Measurement of GTPase, GAP and GEF ActivitiesPoster
We have developed a homogenous bioluminescent assay (GTPase-Glo) system to analyze these proteins in a simple, convenient “add-mix-read” format.READ MORE
Human iPSC-derived cardiomyocytes: A translational model to predict drug-induced cardiac arrhythmias and long-term toxicityPoster
The results shown here imply that iPSC-derived Cor.4U human cardioymocytes are a translational in vitro cell model for the prediction of clinically relevant drug-induced cardiac arrhythmias and long-term toxicity.READ MORE