High Throughput Cell Cycle Analysis using Microplate Cytometry
Poster Feb 01, 2007
Tristan Cope and Wayne Bowen
The cell cycle is a target for many anti-cancer drugs and the ability to monitor the effects of such agents on the cell cycle is an important part of the drug development process. Standard methods measure changes in DNA content by staining the nuclei of fixed cells with propidium iodide. The cells are then sorted by flow cytometry into G1, S and G2/M populations according to fluorescent intensity. The main disadvantages of this technique are low throughput, use of large number of cells and an inability to analyse adherent cell lines in situ.
To improve screening efficiency, we have developed a cell cycle analysis method that uses an Acumen Explorer fluorescence microplate cytometer to measure the DNA content of propidium iodide stained fixed cells in microplates. We demonstrate that paclitaxel and vinblastine arrested CHO cells in the expected phase of the cell cycle.
Assessment of Oral LISPRO Treatment in Ameliorating Amyloid and Tau Pathology in Transgenic Alzheimer’s Mice ModelPoster
Ionic co-crystals of lithium salicylate with organic proline (LISPRO) showed better safety and pharmacokinetic profile of lithium in plasma and brain of wild-type and transgenic Alzheimer mice model compared to lithium salts.READ MORE
CiPA Phase 2 Study: validation of an automated microelectrode array (MEA) assay of hiPSC-derived cardiomyocyte electrophysiology for cardiac safety evaluationPoster
These results support the use of hSC-CM and MEA technology for preclinical assessment of proarrhythmic risk within the proposed CiPA paradigm, and, more generally, demonstrate that automation of the CM-MEA assay can achieve high reliability and throughput for cardiac risk assessment in vitro.READ MORE
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