High Throughput Cell Cycle Analysis using Microplate Cytometry
Poster Feb 01, 2007
Tristan Cope and Wayne Bowen
The cell cycle is a target for many anti-cancer drugs and the ability to monitor the effects of such agents on the cell cycle is an important part of the drug development process. Standard methods measure changes in DNA content by staining the nuclei of fixed cells with propidium iodide. The cells are then sorted by flow cytometry into G1, S and G2/M populations according to fluorescent intensity. The main disadvantages of this technique are low throughput, use of large number of cells and an inability to analyse adherent cell lines in situ.
To improve screening efficiency, we have developed a cell cycle analysis method that uses an Acumen Explorer fluorescence microplate cytometer to measure the DNA content of propidium iodide stained fixed cells in microplates. We demonstrate that paclitaxel and vinblastine arrested CHO cells in the expected phase of the cell cycle.
High Throughput Rheological Characterization of Small Volume Biopharmaceutical FormulationsPoster
Importance of assessing viscosity fingerprints of small molecules.READ MORE
Applications of chemically modified synthetic guide RNA for CRISPR-Cas9 genome editingPoster
Our results indicate that MS modifications are required for experiments with co-electroporation of Cas9 mRNA and synthetic gRNA, yet have no impact on editing efficiency when delivered with lipid-based transfection reagents.READ MORE
Novel lead optimization strategy of BACE I inhibitors for the treatment of Alzheimer’s disease by Quantitative Structure-Activity Relationship (QSAR) and Physiologically-Based Pharmacokinetics (PBPK) modelingPoster
In this study, we introduce an innovative in silico-based high throughput lead optimization strategy with QSAR and PBPK modelings using StarDrop™, ADMET predictor® and GastroPlus®.READ MORE
Comments | 0 ADD COMMENT
Pfizer, Scripps Partner on DNA-Encoded Library Tech
News Jan 11, 2017