High Throughput Rheological Characterization of Small Volume Biopharmaceutical Formulations
Poster Jan 18, 2017
Dr. David Nieto Simavilla, Ivan Akhremitchev, & Dr. Seonggi Baek
During the drug discovery process, biopharmaceutical industries produce a large number of formulations with small recipe variations. One important aspect in the development of novel drugs is the characterization of their viscosity fingerprint (i.e. viscosity dependence on shear rate, temperature…). Viscosity is a good indicator of small changes in concentration and molecular structure that play a key role in the efficacy and stability of the final product.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE