Knockout of microRNAs Using the CRISPR-Cas9 System with Paired Synthetic crRNAs

Poster   Apr 24, 2018

 
Knockout of microRNAs Using the CRISPR-Cas9 System with Paired Synthetic crRNAs
 
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Eldon T. Chou, John A. Schiel, Elena Maksimova, Travis Hardcastle, Emily A. Anderson, Annaleen Vermeulen, Anja van Brabant Smith

 
 

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License

 
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Gene knockout using CRISPR-Cas9 has dramatically transformed biological research and has been rapidly applied to loss-of-function screening primarily using pooled lentiviral sgRNA libraries. A synthetic CRISPR RNA (crRNA) approach is amenable to screening in arrayed, well-by-well fashion and expands the types of phenotypic readouts that can be used, including high-content and morphology-based assays.

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