Miniaturized Kinase uHTS in 1536 Well Format Using IMAP Technology in an Imager
Poster Feb 19, 2007
In this poster we discuss the suitability of an antiboby-independent fluorescence polarization (FP) approach using metal-coated beads (IMAPTM technology) to develop an homogeneous and miniaturized assay for the identification of novel kinase inhibitors from a very large compound collection.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE