Multi Parallel High Pressure Reactions in a 384 Microplate
Poster Apr 05, 2007
The new development of Celisca, the multi-parallel high pressure reactor HPMR 50-384, offers the execution of 384 reactions in a microplate under reaction pressures and temperatures of 50 bar and 100°C. The mixing of the reagents is based on magnetically propelled stir discs. The reactor supplies an inert handling of the substances, can be integrated into laboratory robot systems and offers an LIMS interface.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE