PHERAstar: A Next Generation Multimode Plate Reader for Sophisticated HTS Assays
Poster Jun 19, 2007
The multimode microplate reader PHERAstar from BMG combines the rapid plate reading necessary for HTS with enhanced performance and sensitivity needed to read small liquid volumes along with the flexibility to tackle the most demanding assays. The performance and features of the PHERAstar are presented in this poster with commercially available HTS kits such as HTRF®, AlphaScreen™, and PolarScreen™.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE