Rapid analysis of 3D tumour spheroids in soft agar and on ultra-low attachment plates using a laser scanning imaging system
Poster Feb 19, 2014
Anne F Hammerstein, Diana Caracino, and Paul Wylie
Research to identify new anticancer drugs is currently facing significant challenges, as only 5% of compounds that show efficacy in pre-clinical development go on to become licensed drugs. Traditionally 2D cell culture models have been employed to evaluate drug candidates in the early phases of the drug discovery process, however, there is increasing evidence that cells grown in 2D monolayers do not accurately reflect the biological complexity of tumours. The requirement for better in vitro tumour models that are compatible with high throughput screening campaigns has led to the development of 3D cell cultures models, especially muliticellular tumour spheroids, which retain many of the morphological and genetic traits of tumours.
Here we describe the formation of such spheroids by two methods: On ultra-low attachment plates and in semi-solid agarose. Both methods are compatible with 96- and 384-well microplate formats. We then used the acumen cellista to rapidly image entire microplates (<5 minutes/plate), reporting a range of parameters such as spheroid number, area and volume. The acumen cellista is ideally suited to the high content analysis of spheroids, as the whole-well scanning capability of the instrument will include data from all the spheroids in a well, while the large depth of field of the scan lens allows the determination of individual spheroid volume without the need to acquire a Z stack of images.
Combining a Real-Time In Vitro Cell Viability Assay and RNA Extraction from the Same 3D SpheroidsPoster
We report here the results of using a novel small molecule probe that viable cells convert into a substrate for a shrimp-derived luciferase to generate a signal proportional to the number of viable cells.READ MORE
Performance of the D5000 and High Sensitivity D5000 ScreenTape Assays for the 4200 TapeStation SystemPoster
Here, we focus on quantification, sizing, and sensitivity of both D5000 ScreenTape assays.READ MORE
CiPA Phase 2 Study: validation of an automated microelectrode array (MEA) assay of hiPSC-derived cardiomyocyte electrophysiology for cardiac safety evaluationPoster
These results support the use of hSC-CM and MEA technology for preclinical assessment of proarrhythmic risk within the proposed CiPA paradigm, and, more generally, demonstrate that automation of the CM-MEA assay can achieve high reliability and throughput for cardiac risk assessment in vitro.READ MORE