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Real-Time Monitoring of ATP Depletion Suitable for HTS
Poster

Real-Time Monitoring of ATP Depletion Suitable for HTS

Real-Time Monitoring of ATP Depletion Suitable for HTS
Poster

Real-Time Monitoring of ATP Depletion Suitable for HTS

ATP depletion assays of kinases, ATPases and aminoacyl tRNA synthetases can be performed in the presence of firefly luciferase/luciferin, by monitoring the light emission continuously. Such assays are preferably set up to result in a first order reaction rate with respect to ATP consumption.
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