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Drug Discovery

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Real-Time Monitoring of ATP Depletion Suitable for HTS

Poster

Last Updated: February 1, 2010

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Published: January 30, 2010

Real-Time Monitoring of ATP Depletion Suitable for HTS content piece image

ATP depletion assays of kinases, ATPases and aminoacyl tRNA synthetases can be performed in the presence of firefly luciferase/luciferin, by monitoring the light emission continuously. Such assays are preferably set up to result in a first order reaction rate with respect to ATP consumption.