Transcreener™ KINASE Fluorescence Polarization Assay Performed on the PHERAstar
Poster Oct 16, 2007
Transcreener™ KINASE ASSAY kits are far-red competitive fluorescence polarization immunoassays based on the detection of ADP. Any enzymatic reaction that uses ATP in a range from 1-250 µM can be monitored. With its dual emission detection and five photomultiplier tubes, BMG LABTECH´s PHERAstar provides the speed and sensitivity needed to take full advantage of BellBrook Labs Transcreener™ technology. Furthermore, a specific optical module was developed, thereby making assay setup simple.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE