Universal Homogeneous Bioluminescent Assay To Monitor the Activity of Various Classes of Methyltransferases In Vitro
The assay is universal since it is based on quantitation of S-adenosylhomocysteine(SAH), a product of most methylation reactions, thus can be used with of a broad range of methyltransferases such as DNA, protein, RNA, and small molecules methyltransferases. The bioluminescent assay is quite sensitive and is formatted for HTS applications. In addition, the assay has been validated for all classes of protein methyltransferases (Lysine and Arginine), and with different types of substrates (small peptides, large proteins, or even nucleosomes). A strong feature of this assay is its utility with a broad range of substrates with no limitations their concentration or composition (short vs. long peptides), thus enabling the generation of kinetic data and determining the mechanism of action of various modulators of methyltransferases of interest.