We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
AnaSpec Improves Sensitivity of SensoLyte® pNPP Alkaline Phosphatase Assays
Product News

AnaSpec Improves Sensitivity of SensoLyte® pNPP Alkaline Phosphatase Assays

AnaSpec Improves Sensitivity of SensoLyte® pNPP Alkaline Phosphatase Assays
Product News

AnaSpec Improves Sensitivity of SensoLyte® pNPP Alkaline Phosphatase Assays


Want a FREE PDF version of This Product News?

Complete the form below and we will email you a PDF version of "AnaSpec Improves Sensitivity of SensoLyte® pNPP Alkaline Phosphatase Assays"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

AnaSpec’s SensoLyte® pNPP Alkaline Phosphatase Assay kits now contain a specially formulated pNPP substrate. This new substrate offers the following advantages:

• Ready to use – no need to reconstitute pNPP powder
• Improved sensitivity – detects as low as 0.02 ng alkaline phosphatase
• Improved stability – stores at 4C for one year.

Changes in alkaline phosphatase level and activity are involved in a variety of physiological and pathological events, such as bone development, bone-related diseases, gestation related diseases, inflammatory bowel disease, post-parathyroidectomy stage, and drug toxicity. Alkaline phosphatase is widely used in ELISA for conjugation with secondary antibody and as a reporter for gene expression studies.

These SensoLyte® pNPP Alkaline Phosphatase Assay kits provide a convenient colorimetric assay for detecting alkaline phosphatase in biological samples and in ELISA with alkaline phosphatase conjugated secondary antibody or streptavidin by using colorimetric pNPP (p-Nitrophenyl phosphate) phosphatase substrate. Upon dephosphorylation, pNPP turns yellow and can be detected at absorbance=405 nm.

The SensoLyte® pNPP Alkaline Phosphatase ELISA Assay kits provide an AP-conjugated secondary antibody – goat anti-mouse (cat# 72147-M) or goat anti-rabbit (cat# 72147-R).

AnaSpec also offers a pNPP assay kit for secreted alkaline phosphatase reporter assay, the SensoLyte® pNPP Secreted Alkaline Phosphatase (SEAP) Reporter Gene Assay Kit. A luminometric SEAP kit is also available.
Advertisement