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AnaSpec Improves Sensitivity of SensoLyte® pNPP Alkaline Phosphatase Assays

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AnaSpec’s SensoLyte® pNPP Alkaline Phosphatase Assay kits now contain a specially formulated pNPP substrate. This new substrate offers the following advantages:

• Ready to use – no need to reconstitute pNPP powder
• Improved sensitivity – detects as low as 0.02 ng alkaline phosphatase
• Improved stability – stores at 4C for one year.

Changes in alkaline phosphatase level and activity are involved in a variety of physiological and pathological events, such as bone development, bone-related diseases, gestation related diseases, inflammatory bowel disease, post-parathyroidectomy stage, and drug toxicity. Alkaline phosphatase is widely used in ELISA for conjugation with secondary antibody and as a reporter for gene expression studies.

These SensoLyte® pNPP Alkaline Phosphatase Assay kits provide a convenient colorimetric assay for detecting alkaline phosphatase in biological samples and in ELISA with alkaline phosphatase conjugated secondary antibody or streptavidin by using colorimetric pNPP (p-Nitrophenyl phosphate) phosphatase substrate. Upon dephosphorylation, pNPP turns yellow and can be detected at absorbance=405 nm.

The SensoLyte® pNPP Alkaline Phosphatase ELISA Assay kits provide an AP-conjugated secondary antibody – goat anti-mouse (cat# 72147-M) or goat anti-rabbit (cat# 72147-R).

AnaSpec also offers a pNPP assay kit for secreted alkaline phosphatase reporter assay, the SensoLyte® pNPP Secreted Alkaline Phosphatase (SEAP) Reporter Gene Assay Kit. A luminometric SEAP kit is also available.