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AnaSpec Introduces Line of Secretase Research Solutions
AnaSpec has introduced a line of amyloid-related secretase substrates, assay kits and inhibitors.
Amyloid Precursor Protein, APP, a transmembrane glycoprotein of about 770 amino acids, is cleaved by a-Secretase and b-Secretase to produce C-terminal fragments that are 83 amino acids (C83) and 99-amino acids (C99) in length, respectively.
Subsequent cleavages of C83 and C99 by g-Secretase produce a 3-kD (p3) protein in the former and a 4-kD (b-amyloid) protein in the latter and a CT57-59 in both fragments (reviewed in 1, 2).
The 4-Kd consists of Ab which are 39 to 42 amino acids in length, with Ab 1-42 being the major component of amyloid plaques which accumulates in neurons of Alzheimer’s disease (AD) brain. Consequently, b-Secretase is an important target for AD drug development.
FRET Secretase Substrates:
AnaSpec provides fluorescence resonance energy transfer (FRET) substrates for use in measuring b-Secretase activity.
Upon cleavage of the FRET substrate by b-Secretase, the quenched fluorescence of the donor is released, with the fluorescence signal increasing with b-Secretase activity. Variations include a range of sequence lengths and emission wavelengths.
- Swedish Double Mutation (K670N/M671L)
EnzoLyte™ 520 ß-Secretase Assay Kit: Ready-to-use assay kit for detection of b-Secretase V. 100 assays.
- b -Secretase inhibitors: High-purity peptides that inhibit b-Secretase activity.
- a -Secretase Substrate: A FRET-based a-Secretase substrate, which upon cleavage liberates the Mca (7-Methoxycoumarin-4-acetic acid) fragment from the proximity quenching effect of Dnp (2,4-dinitrophenyl), Abs/Em=325/393 nm.
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