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AnaSpec Introduces new SensolyteO uPA and tPA Assay Kits

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AnaSpec introduces two plasminogen activator assay kits – the SensoLyteO AFC Urokinase (uPA) Assay Kit and the SensoLyteO AMC tPA Assay Kit. These kits, optimized for inhibitor screening, contain high-performance fluorogenic substrates which exhibit high reactivity and low background signal.
The AFC (7-amino-4-trifluoromethylcoumarin) labeled substrate in the SensoLyteO AFC Urokinase (uPA) Assay Kit can be monitored at Ex/Em= 380/500 nm upon cleavage, with increase in fluorescence correlated to uPA activity.
This substrate is not cleaved by tissue-type plasminogen activator (tPA). The AMC (7-amino-4-methylcoumarin) labeled substrate in the SensoLyteO AMC tPA Assay Kit can be monitored at Ex/Em=354/442 nm. Upon protease cleavage, this substrate generates the AMC fluorophore emitting bright blue fluorescence. Increase in AMC fluorescence is proportional to tPA activity.
The AFC (7-amino-4-trifluoromethylcoumarin) labeled substrate in the SensoLyteO AFC Urokinase (uPA) Assay Kit can be monitored at Ex/Em= 380/500 nm upon cleavage, with increase in fluorescence correlated to uPA activity.
This substrate is not cleaved by tissue-type plasminogen activator (tPA). The AMC (7-amino-4-methylcoumarin) labeled substrate in the SensoLyteO AMC tPA Assay Kit can be monitored at Ex/Em=354/442 nm. Upon protease cleavage, this substrate generates the AMC fluorophore emitting bright blue fluorescence. Increase in AMC fluorescence is proportional to tPA activity.