Sign up to read this article for FREE!
After signing up, you'll start to receive regular news updates from us.
AnaSpec Introduces new SensolyteO uPA and tPA Assay Kits
Want to listen to this article for FREE?
Complete the form below to unlock access to ALL audio articles.
Read time: Less than a minute
AnaSpec introduces two plasminogen activator assay kits – the SensoLyteO AFC Urokinase (uPA) Assay Kit and the SensoLyteO AMC tPA Assay Kit. These kits, optimized for inhibitor screening, contain high-performance fluorogenic substrates which exhibit high reactivity and low background signal.
The AFC (7-amino-4-trifluoromethylcoumarin) labeled substrate in the SensoLyteO AFC Urokinase (uPA) Assay Kit can be monitored at Ex/Em= 380/500 nm upon cleavage, with increase in fluorescence correlated to uPA activity.
This substrate is not cleaved by tissue-type plasminogen activator (tPA). The AMC (7-amino-4-methylcoumarin) labeled substrate in the SensoLyteO AMC tPA Assay Kit can be monitored at Ex/Em=354/442 nm. Upon protease cleavage, this substrate generates the AMC fluorophore emitting bright blue fluorescence. Increase in AMC fluorescence is proportional to tPA activity.
The AFC (7-amino-4-trifluoromethylcoumarin) labeled substrate in the SensoLyteO AFC Urokinase (uPA) Assay Kit can be monitored at Ex/Em= 380/500 nm upon cleavage, with increase in fluorescence correlated to uPA activity.
This substrate is not cleaved by tissue-type plasminogen activator (tPA). The AMC (7-amino-4-methylcoumarin) labeled substrate in the SensoLyteO AMC tPA Assay Kit can be monitored at Ex/Em=354/442 nm. Upon protease cleavage, this substrate generates the AMC fluorophore emitting bright blue fluorescence. Increase in AMC fluorescence is proportional to tPA activity.
