We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement

AnaSpec Introduces SensoLyte® NADP/NADPH Assay Kit


Want a FREE PDF version of This Product News?

Complete the form below and we will email you a PDF version of "AnaSpec Introduces SensoLyte® NADP/NADPH Assay Kit"

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

AnaSpec has announced the release of the SensoLyte® Fluorimetric NADP/NADPH Assay Kit.

This assay is a sensitive fluorimetric assay that detects NADP and NADPH without interfering with NAD/NADH. NADP is converted to NADPH in an enzyme-cycling reaction.

The kit contains a fluorogenic reagent, producing fluorescence when reacted with NADPH.

The resulting red fluorescence can be monitored at excitation /emission= 560/590 nm, with the intensity of fluorescence produced being proportional to NADP/NADPH concentration. This assay detects as low as 1.5 nM of the analyte.

Pyridine nucleotides are involved in a number of critical catabolic and anabolic reactions in living organisms. Nicotinamide adenine dinucleotide phosphate (NADP) is a natural coenzyme present in all animal and plant organisms.

Cellular NADPH is important for tolerance to ROS and maintenance of cellular redox homeostasis. The reducing power of NADPH is a required cofactor for enzymes that are prooxidant such as nitric oxide synthase and NADPH oxidase.

Furthermore the NADP+/NADPH ratio has been found to change in the erythrocytes of subjects affected by hemolytic disorder.

In addition to the fluorimetric kit, AnaSpec also provides a convenient colorimetric kit. This kit also detects NADP and NADPH without interfering with NAD/NADH.

The SensoLyte® Colorimetric NADP/NADPH Assay Kit utilizes an enzyme cycling reaction to produce a blue colored product, formazan, which can be monitored at 565 nm using a microplate reader.

The intensity of the color produced is proportional to NADP/NADPH concentration.

Advertisement