AnaSpec Introduces SensoLyte® NADP/NADPH Assay Kit
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AnaSpec has announced the release of the SensoLyte® Fluorimetric NADP/NADPH Assay Kit.
This assay is a sensitive fluorimetric assay that detects NADP and NADPH without interfering with NAD/NADH. NADP is converted to NADPH in an enzyme-cycling reaction.
The kit contains a fluorogenic reagent, producing fluorescence when reacted with NADPH.
The resulting red fluorescence can be monitored at excitation /emission= 560/590 nm, with the intensity of fluorescence produced being proportional to NADP/NADPH concentration. This assay detects as low as 1.5 nM of the analyte.
Pyridine nucleotides are involved in a number of critical catabolic and anabolic reactions in living organisms. Nicotinamide adenine dinucleotide phosphate (NADP) is a natural coenzyme present in all animal and plant organisms.
Cellular NADPH is important for tolerance to ROS and maintenance of cellular redox homeostasis. The reducing power of NADPH is a required cofactor for enzymes that are prooxidant such as nitric oxide synthase and NADPH oxidase.
Furthermore the NADP+/NADPH ratio has been found to change in the erythrocytes of subjects affected by hemolytic disorder.
In addition to the fluorimetric kit, AnaSpec also provides a convenient colorimetric kit. This kit also detects NADP and NADPH without interfering with NAD/NADH.
The SensoLyte® Colorimetric NADP/NADPH Assay Kit utilizes an enzyme cycling reaction to produce a blue colored product, formazan, which can be monitored at 565 nm using a microplate reader.
The intensity of the color produced is proportional to NADP/NADPH concentration.