AnaSpec Launches new SensoLyte™ Caspase-8 Assay Kit
Product News Oct 10, 2007
AnaSpec has introduced its SensoLyte™ Homogeneous AFC Caspase-8 Assay Kit.
The SensoLyte™ Homogeneous AFC Caspase-8 Assay Kit uses Ac-IETD-AFC as the fluorogenic indicator to measure caspase-8 activity. Upon cleavage of the substrate by caspase-8, free AFC (7-amido-4-trifluoromethylcoumarin) emits a yellow-green fluorescence, which can be quantified at excitation/emission=380nm/500nm.
A bi-functional assay buffer in this kit is optimized for cell lysis and measurement of the enzyme activity. This kit can assay caspase-8 activity in cells grown in a 96-well plate or in larger plates or flasks. The kit is adaptable for high throughput screening of apoptosis inducers and inhibitors.