Bio-Rad Releases Poster that Describes Transfection of Primary and Difficult-to-Transfect Cells
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Bio-Rad Laboratories, Inc. has announced the availability of a poster (number 07-0883) that was displayed at the Association for Biomolecular Resource Facilities Annual Meeting held in February, 2008.
In the poster, Bio-Rad demonstrates how high efficiency electroporation of several difficult-to-transfect cell lines was achieved using the parameter optimization capabilities of the Gene Pulser MXcell™ electroporation system.
Electroporation conditions, including optimal waveform and pulse conditions, were determined in parallel for each cell type. Results of several difficult-to-transfect cell types, including HUVEC (human umbilical vein endothelial cell), Jurkat, and mouse neuroblastoma cells (Neuro-2A) with plasmid DNA or siRNA, are shown.
Data presented in the poster shows that four-hours post electroporation with a GAPDH siRNA, HUVEC cells expressed less than 97% of GAPDH mRNA than controls transfected with scrambled siRNA. In Jurkat cells, significant silencing was also obtained (>88%) as quickly as four hours post transfection and this silencing persisted for 48 hours. Transfection of Neuro-2A cells with a fluorescently labeled siRNA followed by analysis with flow cytometry resulted in 75% transfection efficiency.
