We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
Easy-to-use, Quantitative Gamma-H2AX Assay
Product News

Easy-to-use, Quantitative Gamma-H2AX Assay

Easy-to-use, Quantitative Gamma-H2AX Assay
Product News

Easy-to-use, Quantitative Gamma-H2AX Assay


Want a FREE PDF version of This Product News?

Complete the form below and we will email you a PDF version of "Easy-to-use, Quantitative Gamma-H2AX Assay"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

AMSBIO has announced the first commercially available gamma H2AX Pharmacodynamic assay kit for the study of double strand DNA breaks through the detection of gamma H2AX - a phosphorylated histone historically proven as a highly specific and sensitive molecular marker for double strand DNA damage detection.

This new assay has been developed for anti-cancer drug screening, basic research and upcoming clinical trials providing one of many needed tools to support hypothesis-driven drug design strategies.

Documented variability in DNA double-strand break repair among different segments of the human population may contribute to patient specific therapeutic toxicities, enabling a more personalized approach to treatment.

A quantitative determination of gamma-H2AX levels in response to treatment would help to establish patient specific dose regimens minimizing the toxicity, while maximizing the efficacy of therapy.

Until now, induction of gamma-H2AX has been determined by either Western Blots or scoring foci with Immunofluorescence. While Western Blots provide information regarding the gamma-H2AX protein, they are difficult to quantitate and provide mostly qualitative data.

Although image analysis software enables foci quantification, actual gamma-H2AX levels cannot be determined by these techniques, as there is no direct relationship between foci and gamma-H2AX levels after damage.

AMSBIO's new 96-well non-radioactive ELISA assay documents differences in gamma H2AX levels in peripheral blood mononuclear cells, cultured cells, and tissue biopsies and is available as a complete reagent kit with chemiluminescent detection.

Alexei Degretev from Tufts University (Medford, USA), one of the initial beta testers of the product, commented "This new gamma H2AX pharmacodynamic assay is a very useful tool for providing precise quantitation of gamma H2AX formation. The assay is easy to perform, fast, and displays good linear range and excellent reproducibility between replicate samples".

Advertisement