Greiner Bio-One Develops Platform for Production and Analysis of Protein Crystals
Product News Aug 04, 2009
Greiner Bio-One GmbH has developed a new platform for the production and analysis of protein crystals. CrystalSlide for counter diffusion application enables crystals to be analyzed in an X-ray source and is also suitable for applications with polarized and UV light.
A key prerequisite for targeted drug development is knowing the three-dimensional structure of proteins. In order for this to be possible, however, the proteins must be available in the form of high-quality pure crystals.
Greiner Bio-One GmbH has now developed a new platform for counter diffusion applications; that is to say for the production of protein crystals in a concentration gradient; in a handy slide format.
CrystalSlide has twelve microstructured channels. The design has been optimized to ensure that the inlets are suitable for manual filling using a pipette and the slides can be used in automated systems – in conjunction with a frame in standard microplate format.
The benefits of CrystalSlide are not limited to the production of protein crystals. In addition to enabling its valuable contents to be analyzed under any conventional microscope, CrystalSlide is also suitable for all commonly used automated imaging systems. At 0.6 millimetres thickness, the polyolefin slides combine optical characteristics with low birefringence and are permeable to UV light and X-rays for in-situ analysis of protein crystals. The low sample volume required also helps cut the cost of protein crystallization processes.
CrystalSlide™ is part of Greiner Bio-One GmbH’s CrystalStar™ family, which includes a number of protein crystallization platforms and associated accessories.