Nanotoxicology Identifies NanoSight's LM Systems for Nanoparticle Sizing

NanoSight Limited, the nanoparticle characterization company, reports growth in sales of the LM series of bench-top system for sizing and counting of individual nanoscale particles in suspension.

One of the most important emerging nanoscience areas is nanotoxicology. In this rapidly growing science, the characterization of nanoparticles, including their size distribution in a biological medium is critical. Average size is not enough, as the smaller particles in a wide dispersion could well be the ones to enter and affect cells.

Professor Kenneth Dawson of the Department of Chemistry at University College Dublin has recently led a European Science Foundation symposium in Barcelona to investigate the interactions between nanoparticles/biomaterials and biological systems. This emerging topic attracted over 150 participants to look into alternative approaches to bio- and nano-safety.

Professor Dawson's work has been greatly enhanced since he started to use the NanoSight nanoparticle sizing system.

"Whist we still use dynamic light scattering to find average particle size, we really need the complete particle distribution map in the sub-micron area in our work. The NanoSight instrument identifies and tracks individual particles, enabling us to see how they are organized (into clusters or otherwise) for the first time. It is impressive to see just how limited DLS is in these respects, where it will often smear or mask true cluster distribution."

NanoSight's systems offer the ability to obtain higher resolution particle size distribution profiles from samples with minimal sample pre-treatment.

After simply diluting to an acceptable concentration range, the instrument uses a patented laser illumination method to visualize individual nanoscale particles moving under Brownian motion. The system recognizes and quantifies polydispersed and multimodal samples as well as agglomerates and contaminants.

The Nanoparticle Tracking Analysis (NTA) analytical software package directly measures the dynamic behavior and, thus, hydrodynamic size of each particle in a suspension and avoids the problems associated with the intensity bias to larger particles inherent in other bulk measurement dynamic light scattering techniques. The software enables real-time dynamic nanoparticle visualization from which independent quantitative estimation of particle size and size distribution can be obtained.