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Protocol for Preparing Top Quality Lyophilised Samples


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Genevac has developed a protocol using its SuperCool 75 condenser that, in conjunction with the Genevac HT-12 High Throughput Evaporator, has been proven to set a new benchmark for enhanced evaporation and lyophilisation results.

The SuperCool 75 chills to -75C and operates at higher power than any other commercially available condenser, opening the door to greatly improved results with the Genevac LyoSpeed fast lyophilisation process for preparing powdered samples from HPLC fractions. Customer trials have shown that the Genevac HT-12 Evaporator / SuperCool 75 combination produces powdered lyophilisation samples using the LyoSpeed methodology from even the most difficult to handle aqueous / organic solutions with a success rate as high as 96%.

Drying HPLC purification fractions, principally comprising water and acetonitrile, is a routine yet essential task in many pharmaceutical laboratories. The requirement is to dry the samples to a powder form, such that samples can be accurately weighed, easily sub-sampled and redissolved.

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