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Ready to Use Disposable 96-Sample Equilibrium Dialysis Plates
Read time: Less than a minute
Harvard Apparatus offers patented 192-Well DispoEquilibrium DIALYZERSTM that provide simple and reliable methods to analyze up to 96 samples simultaneously on disposable dialysis plates, available only from Harvard Apparatus.
These ready to use equilibrium dialysis plates are effective tools for ligand binding experiments including serum protein binding, protein-drug binding, protein-protein binding and protein-DNA binding assays.
Equilibrium Dialysis is recognized as the Gold Standard or the most preferred technique for molecular binding studies as it allows for the direct assays of molecular interactions at close to physiological conditions with minimal effects on equilibrium parameters.
Equilibrium Dialysis has also shown itself to be a valuable technique for sample preparation before candidate analysis by mass spectrometry. Studies have shown enhanced target identification when equilibrium dialysis is used.
Each test well in the plate consists of two chambers separated by a regenerated cellulose membrane with a molecular weight cut off of either 5000 or 10000 Daltons. Each chamber holds up to 300µl of sample or buffer.
To decrease the dialysis time, the plate needs to be rotated through 360° in a vertical position. Harvard Apparatus can also supply a 1 or 2 plate rotator or an eight plate rotator with temperature control.
The 96–well plate was designed with SBS footprint and well spacing to meet automation needs.
Other equilibrium dialysis products from Harvard Apparatus include the single-sample DispoEquilibrium dialyzer for volumes up to 100µl or the re-usable PTFE Fast Micro-Equilibrium dialyzer for samples from 25µl to 1500µl and the Multi-Equilibrium Dialysis System for up to 20 samples with volumes from 200µl to 5ml.
These ready to use equilibrium dialysis plates are effective tools for ligand binding experiments including serum protein binding, protein-drug binding, protein-protein binding and protein-DNA binding assays.
Equilibrium Dialysis is recognized as the Gold Standard or the most preferred technique for molecular binding studies as it allows for the direct assays of molecular interactions at close to physiological conditions with minimal effects on equilibrium parameters.
Equilibrium Dialysis has also shown itself to be a valuable technique for sample preparation before candidate analysis by mass spectrometry. Studies have shown enhanced target identification when equilibrium dialysis is used.
Each test well in the plate consists of two chambers separated by a regenerated cellulose membrane with a molecular weight cut off of either 5000 or 10000 Daltons. Each chamber holds up to 300µl of sample or buffer.
To decrease the dialysis time, the plate needs to be rotated through 360° in a vertical position. Harvard Apparatus can also supply a 1 or 2 plate rotator or an eight plate rotator with temperature control.
The 96–well plate was designed with SBS footprint and well spacing to meet automation needs.
Other equilibrium dialysis products from Harvard Apparatus include the single-sample DispoEquilibrium dialyzer for volumes up to 100µl or the re-usable PTFE Fast Micro-Equilibrium dialyzer for samples from 25µl to 1500µl and the Multi-Equilibrium Dialysis System for up to 20 samples with volumes from 200µl to 5ml.
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