Recombinant Protein Fermentation Monitoring in Real Time
Product News Feb 04, 2011
The application note, Rapid Analysis of Recombinant Protein Production During Fermentation Using Dionex ProPac SCX Columns, details how the ProPac® SCX-10 column can be used for real-time monitoring of recombinant protein production, with reduced analysis time and smaller sample volume requirements. Further time savings can be achieved using the ProSwift® SCX-1S column. This customer application note explains how to purify and analyze recombinant lysostaphin directly from cell-free lysate without any additional purification steps; this approach is advantageous as it permits rapid monitoring as the fermentation progresses. This method also demonstrates that small volumes of culture (as little as 1 mL) can provide sufficient material for rapid analysis.