Teach your GPCR to behave: simple solutions to common Gq behavior problems
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The proper optimization of assay conditions is essential to ensure you obtain the best reagent use and performance. In the case of GPCR signaling, the most suitable assay conditions depends on the GPCR coupling (Gq) efficacy as well as the pharmacological characteristics of the compound being studied (agonist or antagonist). The optimal assay conditions will also hinge on the particular cell line used for the assay. For each setup, a number of optimization steps can be implemented that will ensure the best, most accurate results are obtained. Drawing on hundreds of data sets and cases established by Cisbio’s scientists over the years, this manual provides the GPCR community with the most up to date guidelines for the optimization of Gαq GPCR assays.