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XPressMAX™ Cell-Free Protein Synthesis Kit: Expression Completed in 3 Hours
Leveraging years of expertise and a proven track record in cell-free protein synthesis, Sino Biological has developed the XPressMAX™ Cell-Free Protein Synthesis Kit (Cat#: CFKIT02). This kit provides an efficient and flexible in vitro solution for rapid protein expression, high-throughput screening, and the accelerated validation of AI-designed proteins.
Utilizing optimized E. coli lysates, the system includes all necessary enzymes, energy regeneration components, and proprietary buffers required for high-efficiency transcription and translation. By eliminating the need for cell culture or transfection, researchers can simply add plasmid or PCR templates to produce highly soluble, active proteins within hours.
The XPressMAX™ system is engineered for antibody discovery, de novo protein design, and the iterative optimization of AI models. It is particularly effective for the high-throughput screening of VHH, scFv, Fab, and miniproteins, as well as the production of toxic or hard-to-express proteins that often fail in conventional prokaryotic or eukaryotic systems.
XPressMAX™ Highlights:
• Ultra-Fast Synthesis:
Protein expression completed in just 3 hours.
• High Success Rate: >99%
Optimized for rapid expression of VHH, scFv, and miniproteins with unparalleled fidelity, significantly improving candidate screening efficiency.
• Broad Template Compatibility
Supports both plasmid DNA and linear DNA (PCR products) as expression templates, providing flexibility for different experimental stages.
• Supports Complex Disulfide Bonding
Efficiently produces proteins with complex disulfide bonds without additional enhancers, ensuring proper folding and preserving native structure and biological activity.
• Highly Cost-Effective
Delivers comparable activity to traditional cell-based systems while offering significant cost advantages.
• Industrial-Scale Supply Capability
Available in bulk formats to seamlessly support high-throughput screening and enable scalable protein production.
