Flow Cytometry – Multimedia

This online tool guides you through flow cytometry panel design, providing a simplified, customizable experience to fit your flow cytometry panel design needs.

Antibodies are used in a broad range of research and diagnostic applications for the enrichment, detection, and quantitation of proteins and their modifications.

The CRISPR interference (CRISPRi) machinery is an efficient, reversible and specific genome regulating platform for transcription control, without altering the target DNA sequence. This guide includes key steps on implementing CRISPRi using dCas9 derived from Streptococcus pyogenes.

The new CellStream™ benchtop flow cytometer delivers unparalleled sensitivity and flexibility for cell and particle analysis.

Developed initially in the field of immunology, flow cytometry now has applications throughout the biological sciences and beyond. A successful flow cytometry experiment begins with good sample preparation.

Angioimmunoblastic T-cell lymphoma (AITL) is a common type of peripheral T-cell lymphoma. AITL can be missed until lymphadenopathy develops in patients initially presenting with skin lesions, as skin biopsy may lack conclusive findings. Our case highlights the extranodal presentation of AITL with cutaneous lesions displaying the TFH phenotype.

We found a distinct subpopulation of Tregs within BMSCs. Tregs and BMSCs in co-culture conferred neuroprotection that varied in a dose-dependent manner. Tregs minimized stem cell production of IL-6, a pro-inflammatory cytokine, and inhibited BMSC secretion of FGF-beta, a cytokine related to BMSC proliferation and differentiation. The ratio of Tregs found natively in BMSCs is optimally adapted to provide the maximum neuroprotective benefit of stem cell treatment after ischemic stroke.

Patients with partial deficiency of recombination-activating genes 1 or 2 (RAG1/2) can present with a wide spectrum of primary immunodeficiencies including combined immunodeficiency with granuloma and/or autoimmunity (CID-G/AI). Prior case reports have highlighted alterations in B and T cell compartments; however comprehensive characterization of these cell populations with focus on autoreactive-prone subsets has not been reported.

The following is a list of what I think are the most useful tips for successful transfection, learned through years of experience and troubleshooting transfection experiments.

Here are 10 tips to help you optimize your clinical flow cytometry experiments and ensure consistent results.