We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience, read our Cookie Policy

SPR Imaging-based Microarray for Enhanced Multianalyte Detection in Blood Plasma

Video   Jul 07, 2015

 



About the SpeakerResearch Assistant, Institute of Photonics and Electronics ASCR, v.v.i., Prague, Czech Republic2010 - present: Doctoral Study: Charles University, Faculty of Mathematics and Physics, Department of Biophysics Thesis: Multifunctional biomolecular systems for parallelized analysis of biomolecular interaction2007 - 2009: Master Study: Charles University, Faculty of Science, Department of Biochemistry Thesis: Study of biomolecular interactions by the method of surface plasmon resonance2004 - 2007: Bachelor study: Charles University, Faculty of Science, Department of Biochemistry Thesis: Optical biosensors and study of biomolecular interactions.
AbstractThis work reports on a novel biosensor allowing for ultra-sensitive multianalyte detection in blood plasma. The biosensor combines a surface plasmon resonance (SPR) imaging-based microarray assay with biofunctionalized gold nanoparticles (AuNPs) for the sensitive and label-free detection of cancer biomarkers. Passive mixing structures are employed to further improve the sensing performance of this SPR imaging biosensor. Preliminary results have shown that the AuNPs increase the contrast between the specific and non-specific binding, which presents major issue in SPR biosensor-based analysis of complex samples (e.g. blood plasma), and thus allow increasing sensitivity and specificity of the biosensor. The passive mixing structures provide significant rates of fluid mixing within the flow channel, enhancing analyte binding rates and improving homogeneity of sensor response along the multi-spot array. The results of this work can be used to significantly enhance the detection capabilities of SPR imaging-based microarrays, regardless of the analyte and sample matrix in question.

 
 
 
 

Recommended Videos

Webinar: Improving hepatocyte activity, pooling and lab efficiency

Video

CryostaX® hepatocytes are created using a patented process that produces unique single-donor cell pellets. This format allows for distinct benefits to hepatocyte performance, efficiency in the lab, and test system design, precipitating the potential discontinuation of cryopreserved hepatocytes prepared from traditional methods. This webinar will discuss the technology, its optimal utilization and benefits

WATCH NOW

Signals Notebook: Finally More Time For Science

Video

PerkinElmer Signals Notebook allows you to achieve meaningful scientific breakthroughs – faster and smarter.

WATCH NOW

What is Leukemia?

Video

Stem cells found in the bone marrow are crucial for our health because they are needed to become new blood cells that sustain and protect our bodies. But when the transformation goes wrong, harmful mutations can cause the cells to start replicating without control -- a type of cancer known as leukemia.

WATCH NOW

 

Like what you just watched? You can find similar content on the communities below.

Applied Sciences Diagnostics

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE