Designing In Vitro Assays for Better Antibody-Drug Conjugate Selection

As antibody-drug conjugate (ADC) development becomes increasingly competitive, many candidates fail to translate effectively from in vitro findings to in vivo studies. This webinar explores how thoughtful in vitro assay design, such as cytotoxicity, bystander killing, internalization, and serum stability studies, can address key challenges, including limited translational insight, narrow therapeutic windows, and candidate differentiation. Through practical examples and case-based discussion, attendees will gain a clearer understanding of how to evaluate mechanism of action, specificity and potency, and [ZG1.1]generate robust, decision-ready data that strengthens confidence in ADC discovery and improves the likelihood of downstream success. 1. Understand how to design in vitro models and select appropriate cell lines to differentiate new ADC candidates 2. Learn how to apply core assays to define and optimize therapeutic windows 3. Gain insight into how advanced analytical tools, including high-content imaging, flow cytometry, and IncuCyte, can be used to generate robust, quantitative data 4. Discover how to translate assay results into actionable insights that bridge discovery and translational studies