Differential Scanning Calorimetry: Theory and Practice

Here the thermodynamic background to DSC measurements is discussed and its application in studying the stability of proteins both alone and interacting with ligands.

The key benefit with DSC is that it is based on heat measurements and therefore allows the characterization of native biomolecules. Furthermore, the lack of spectroscopic readings means that the samples do not have to be optically clear. In addition, the characterization is not limited to the melting temperature (Tm), but it also provides data on the forces involved in folding of biomolecules and the mechanisms by which they unfold.