Measuring the concentration of Adeno-Associated Virus with multi-angle dynamic light scattering (MADLS)
Dynamic light scattering (DLS) is often used in virus development, in a screening function to separate good and stable samples from those with contaminants or aggregates. With the new multi-angle DLS (MADLS) based particle concentration measurement in the Zetasizer Ultra, it is now possible to get both size and size distribution as well as particle concentration per population, within a few minutes.
In this application note, Allergan are kindly sharing data from their evaluation of the Zetasizer Ultra, examples of three adeno-associated virus (AAV) samples are shown. The concentration results are compared to results from capsid ELISA based virus titre assays.