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Application Notes

 
A Fast and Simple Method For Measuring P-Glycoprotein (Pgp) Inhibition
Application Note

BMG Labtech

Pgp expression has been linked to the effl ux of chemotherapeutic
drugs in human cancers leading to multidrug resistance. Pgp
activity can also result in low oral absorption and poor brain
penetration. Interaction of drugs with the Pgp may also cause
an increase in toxicity of co-administered compounds.

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Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA Microplate Reader
Application Note

BMG Labtech

The detection of SNPs (single nucleotide polymorphisms) is an important tool for researchers in order to find mutations in DNA sequences. In this application note we describe a homogeneous fluorescence assay based on PCR. The Amplifluor® SNPs HT Genotyping System from Millipore was used to screen a number of samples with the help of the FLUOstar OPTIMA multidetection microplate reader from BMG LABTECH.

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High Yield and Quality with Thermo Scientific Microtiter Deep Well 96 plates in KingFisher Flex Process
Application Note

The Thermo Scientific KingFisher Flex magnetic particle processor is specifically designed to automate the time-consuming sample preparation of proteins, nucleic acids and cells in 96-well plate formats.

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Isolation and Purification of Genomic DNA and Viral RNA from Clinical Samples with Thermo Scientific KingFisher Flex
Application Note

The purification of DNA and RNA from clinical samples requires rapid and reliable processing of large quantities of samples. Automated purification of nucleic acids, proteins and cells with Thermo Scientific KingFisher Flex is a reproducible and high quality method, which produces excellent material for further analyses.

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DNA Quantitation in Cuvette and TrayCell using the Thermo Scientific Multiskan GO Spectrophotometer
Application Note

This application note describes how to perform photometric DNA quantitation and qualification measurements with the Thermo Scientific Multiskan GO microplate and cuvette spectrophotometer in various cuvette formats.

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Rapid and Reproducible DNA Isolation from 1 ml of Whole Blood with Thermo Scientific KingFisher Flex
Application Note

This application note shows the benefits of KingFisher Flex by using genomic DNA isolation from blood as an example.

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Device Qualification of the Thermo Scientific Finnpipette Novus Electronic Pipette (IQ/OQ/PQ)
Application Note

During device qualification, one verifies and documents whether the equipment, in this case a pipette, is reliable and capable of fulfilling the requirements of its intended use. All qualification activities are fully documented according to pertinent quality and regulatory requirements and signed by authorized personnel.

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Use of Thermo Scientific Finnpipette Novus Electronic Pipettes for Accurate and Efficient Preparation of Standard Curves
Application Note

This application note discusses the use of a Thermo Scientific Finnpipette Novus Electronic Pipette for the preparation of standard curves. With 10 different functions, the versatile Novus Electronic pipette can perform most every standard curve required. The pipette’s performance was compared to a manual Thermo Scientific Finnpipette model to determine if there were appreciable differences in pipetting speed and accuracy during curve preparation.

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Validated Luminometric DLR™ Reporter Gene Assay with Thermo Scientific Varioskan® Flash
Application Note

One of the most common luminometric assays is Dual Luciferase® Reporter Assay from Promega corp. This application note shows how this assay is performed with Varioskan Flash multitechnology microplate reader. The note includes the performance of the normal DLR assay and results from some tests showing important aspects of the basic behaviour and performance of the DLR™ chemistry. This paper describes the overall efficiency of the Varioskan Flash in the measurement of DLR assay.

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Optimization of the DNA Purification Protocol for the Thermo Scientific KingFisher Flex and BindIt 3.1 Software
Application Note

Purpose: Optimal quantity and quality of DNA with the magnetic particle based extraction technology. Method: Thermo Scientific KingFisher Flex magnetic particle processor and BindIt® 3.1 Software. Results: By simple optimization of the nucleic acid extraction protocol it is possible to achieve a great yield of high quality DNA.

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