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Application Notes

 
Automated Illumina NGS Sample Preparation: TruSeq® Stranded mRNA on the Hamilton Microlab STAR
Application Note

Illumina’s sample preparation kits produce high quality libraries for DNA and RNA sequencing. Hamilton offers the robust Microlab STAR line of robotic liquid handling workstations for automating sample preparation. Together, the automation of the TruSeq Stranded mRNA sample preparation protocol on the STAR gives the user hours of reliable, unattended library preparation.

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Molecular indexing for improved RNA-Seq analysis
Application Note

Bioo Scientific

Most modern methods for nucleic acid analysis require the use of enzyme processing, such as DNA polymerase reactions, in the sample preparation or measurement steps. Although necessary, these enzymatic steps introduce errors in the form of incorrect sequence and misrepresented copy number. NEXTflex™ qRNA-Seq™ Kit from Bioo Scientific adds no additional steps to the work flow, costs no more than a conventional library preparation kit and increases the precision of downstream analysis.

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An Integrated Solution to Simplify Library Preparation and Multiplexing for NimbleGen Sequence Capture
Application Note

Bioo Scientific

The studies described herein demonstrate the capability of Bioo Scientific’s novel NEXTFlex™ DNA Pre-Capture Combo Library construction kits used in conjunction with the NimbleGen Sequence Capture technology to obtain high coverage comparative genomic data from a panel of human HapMap DNA samples.

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BRD4 Bromodomain 1 Inhibitor Screening Using a Homogeneous Proximity Assay
Application Note

BioTek Instruments Inc.

Demonstration of profiling and primary screening of an epigenetic target; a histone acetylating bromodomain. This is a TR-FRET based application highlighting the use of automated instrumentation and a high-throughput screening (HTS) multi-mode microplate reader.

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Purification of Genomic DNA from Saliva Using Oragene® Discover Collection Kits and the Akonni TruTip® Extraction System on the Hamilton Microlab® STAR Liquid Handling Workstation
Application Note

Hamilton

In the following studies, we have demonstrated the high precision of the automated TruTip extraction technology in processing genomic DNA from low-volume saliva samples and its advantage over competitors.

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Rapid, High Throughput Extraction and Purification of Genomic DNA from Whole Blood Using the Akonni TruTip® Extraction System on the Hamilton Microlab® STAR Liquid Handling System
Application Note

Hamilton

This application note demonstrates the high precision of the automated extraction system in processing genomic DNA from low volume whole blood samples and its advantage over competitors.

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Next Generation Sequencing Method for Illumina TruSeq DNA Sample Preparation Protocol on the Hamilton STAR
Application Note

Hamilton

The purpose of the TruSeq sample preparation protocol is to add adapter sequences onto the ends of DNA fragments to generate multiplexed sequencing libraries. Good liquid handling is crucial for pipetting precise volumes for example for Agilent Bioanalyzer.

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Automated GS FLX Titanium emPCR Enrichment using the REM e System on a Hamilton Microlab® STARlet Liquid Handler
Application Note

Hamilton

The Genome Sequencer FLX System is a versatile sequencing platform suitable for a wide range of research applications, including de novo sequencing and assembly of genomic DNA, transcriptome sequencing, small RNA analysis, and amplicon sequencing.

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A Fluorescence Based Assay of the Epigenetic Enzyme Histonedeacetylase 1 (HDAC1)
Application Note

BMG Labtech

Post-translational histone modifications like acetylation play a pivotal role in the epigenetic regulation of transcription. Catalyzing the latter reaction HDACs affect various cellular processes especially cancerogenesis. Although the mechanism of starting cancerogenesis by epigenetic events is not clearly explained inhibition of HDACs has highlighted as a viable principle in cancer therapy.

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Analysis of Organophosphorus Pesticides with Agilent 7820 Gas Chromatograph Flame Photometric Detector
Application Note

The Agilent 7820 series gas chromatograph (GC) equipped with a flame photometric detector (FPD) provides high sensitivity, good linearity, and stability for the analysis of organophosphorus pesticides.

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