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Application Notes

 
Isolation and Purification of Genomic DNA and Viral RNA from Clinical Samples with Thermo Scientific KingFisher Flex
Application Note

The purification of DNA and RNA from clinical samples requires rapid and reliable processing of large quantities of samples. Automated purification of nucleic acids, proteins and cells with Thermo Scientific KingFisher Flex is a reproducible and high quality method, which produces excellent material for further analyses.

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DNA Quantitation in Cuvette and TrayCell using the Thermo Scientific Multiskan GO Spectrophotometer
Application Note

This application note describes how to perform photometric DNA quantitation and qualification measurements with the Thermo Scientific Multiskan GO microplate and cuvette spectrophotometer in various cuvette formats.

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Rapid and Reproducible DNA Isolation from 1 ml of Whole Blood with Thermo Scientific KingFisher Flex
Application Note

This application note shows the benefits of KingFisher Flex by using genomic DNA isolation from blood as an example.

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Device Qualification of the Thermo Scientific Finnpipette Novus Electronic Pipette (IQ/OQ/PQ)
Application Note

During device qualification, one verifies and documents whether the equipment, in this case a pipette, is reliable and capable of fulfilling the requirements of its intended use. All qualification activities are fully documented according to pertinent quality and regulatory requirements and signed by authorized personnel.

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Use of Thermo Scientific Finnpipette Novus Electronic Pipettes for Accurate and Efficient Preparation of Standard Curves
Application Note

This application note discusses the use of a Thermo Scientific Finnpipette Novus Electronic Pipette for the preparation of standard curves. With 10 different functions, the versatile Novus Electronic pipette can perform most every standard curve required. The pipette’s performance was compared to a manual Thermo Scientific Finnpipette model to determine if there were appreciable differences in pipetting speed and accuracy during curve preparation.

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Validated Luminometric DLR™ Reporter Gene Assay with Thermo Scientific Varioskan® Flash
Application Note

One of the most common luminometric assays is Dual Luciferase® Reporter Assay from Promega corp. This application note shows how this assay is performed with Varioskan Flash multitechnology microplate reader. The note includes the performance of the normal DLR assay and results from some tests showing important aspects of the basic behaviour and performance of the DLR™ chemistry. This paper describes the overall efficiency of the Varioskan Flash in the measurement of DLR assay.

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Optimization of the DNA Purification Protocol for the Thermo Scientific KingFisher Flex and BindIt 3.1 Software
Application Note

Purpose: Optimal quantity and quality of DNA with the magnetic particle based extraction technology. Method: Thermo Scientific KingFisher Flex magnetic particle processor and BindIt® 3.1 Software. Results: By simple optimization of the nucleic acid extraction protocol it is possible to achieve a great yield of high quality DNA.

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Complementing Next Generation Sequencing Technologies With Agilent’s SureSelect DNA Capture Array
Application Note

Agilent Technologies

Massively parallel DNA sequencing technologies are of pivotal importance in genome biology and medicine, as they can potentially enable comprehensive and systematic evaluation of genetic variation. Currently, these sequencing technologies are geared toward sequencing whole genomes.

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Simultaneous Detection of Copy Number and Copy-Neutral LOH Using a Single Microarray
Application Note

Agilent Technologies

Application note describing the Agilent SurePrint G3 CGH+SNP microarray platform for the simultaneous detection of copy number and copy-neutral genetic aberrations.

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Mitos P-Pump Droplet Monodispersity
Application Note

Dolomite

The formation of droplets of oil-in-water or water-in-oil has a range of uses in science and industry. Monodispersity, or size consistency, is the key to making droplet microfluidics a powerful tool for conducting accurate and repeatable experiments.

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