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Latest App Notes & Case Studies

App Note / Case Study

Device Qualification of the Thermo Scientific Finnpipette Novus Electronic Pipette (IQ/OQ/PQ)

During device qualification, one verifies and documents whether the equipment, in this case a pipette, is reliable and capable of fulfilling the requirements of its intended use. All qualification activities are fully documented according to pertinent quality and regulatory requirements and signed by authorized personnel.
App Note / Case Study

Use of Thermo Scientific Finnpipette Novus Electronic Pipettes for Accurate and Efficient Preparation of Standard Curves

This application note discusses the use of a Thermo Scientific Finnpipette Novus Electronic Pipette for the preparation of standard curves. With 10 different functions, the versatile Novus Electronic pipette can perform most every standard curve required. The pipette’s performance was compared to a manual Thermo Scientific Finnpipette model to determine if there were appreciable differences in pipetting speed and accuracy during curve preparation.
App Note / Case Study

Validated Luminometric DLR™ Reporter Gene Assay with Thermo Scientific Varioskan® Flash

One of the most common luminometric assays is Dual Luciferase® Reporter Assay from Promega corp. This application note shows how this assay is performed with Varioskan Flash multitechnology microplate reader. The note includes the performance of the normal DLR assay and results from some tests showing important aspects of the basic behaviour and performance of the DLR™ chemistry. This paper describes the overall efficiency of the Varioskan Flash in the measurement of DLR assay.
App Note / Case Study

Optimization of the DNA Purification Protocol for the Thermo Scientific KingFisher Flex and BindIt 3.1 Software

Purpose: Optimal quantity and quality of DNA with the magnetic particle based extraction technology. Method: Thermo Scientific KingFisher Flex magnetic particle processor and BindIt® 3.1 Software. Results: By simple optimization of the nucleic acid extraction protocol it is possible to achieve a great yield of high quality DNA.
App Note / Case Study

Complementing Next Generation Sequencing Technologies With Agilent’s SureSelect DNA Capture Array

Massively parallel DNA sequencing technologies are of pivotal importance in genome biology and medicine, as they can potentially enable comprehensive and systematic evaluation of genetic variation. Currently, these sequencing technologies are geared toward sequencing whole genomes.
App Note / Case Study

Simultaneous Detection of Copy Number and Copy-Neutral LOH Using a Single Microarray

Application note describing the Agilent SurePrint G3 CGH+SNP microarray platform for the simultaneous detection of copy number and copy-neutral genetic aberrations.
App Note / Case Study

Mitos P-Pump Droplet Monodispersity

The formation of droplets of oil-in-water or water-in-oil has a range of uses in science and industry. Monodispersity, or size consistency, is the key to making droplet microfluidics a powerful tool for conducting accurate and repeatable experiments.
App Note / Case Study

Monitoring Bacterial Cell-to-Cell Communication “Quorum sensing” Using FLUOstar OPTIMA

Bacteria can monitor and respond to changes to environmental conditions via a cell density process known as quorum sensing. Bacteria use this process to monitor their community by producing, detecting and responding to low molecular mass signal molecules, called autoinducers. Effects on quorum sensing were investigated by measuring growth and bioluminescence of Vibrio fischeri. The FLUOstar OPTIMA was utilized to measure absorbance and luminescence in parallel.
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