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Application Notes

 
Reduced-Bias Small RNA Library Preparation with Gel-Free or Low-Input Options
Application Note

Bioo Scientific

Changes in microRNA (miRNA) expression have been shown to be associated with a variety of normal physiological processes, as well as diseases including cancer. Studies have already shown that miRNAs may provide useful markers for the development of disease diagnostic and prognostic assays.

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Characterization of the FlexJet® with Multi-Array Dispense for Reduction of Chemistry Waste Due to Overage
Application Note

Douglas Scientific

This study demonstrates that the Nexar® with the FlexJet multi-array dispense feature can reduce master mix consumed as dead volume by 87.5% compared to Dispense Jet single-array dispense. The capability to perform multi-array dispense with one aspiration also produces repeatable and accurate results and is an ideal solution for genotyping applications.

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Multiplex Genotyping of Human Reference DNA Samples with Pharmacologically Important Single Nucleotide Polymorphisms using the IntelliQube® from Douglas Scientific
Application Note

Douglas Scientific

This study demonstrates the ability of the IntelliQube along with Array Tape®, to successfully achieve two multiplexed SNP genotyping results in a single well and decrease thermal cycling times without compromising data quality. The combination of achieving two results per reaction well and shortened cycling times dramatically increases overall throughput.

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Muliplex SNP Genotyping of Field Corn Crude Samples with Probe-Based Assays using the IntelliQube® from Douglas Scientific®
Application Note

Douglas Scientific

This study evaluated multiplex genotyping on the IntelliQube, a PCR instrument optimized for 1.6 µL reaction volumes in Array Tape®. It demonstrates the feasibility of achieving two SNP genotyping results in a single reaction well. IntelliQube provides cost savings through miniaturized PCR reaction volumes, shorter thermal cycling times, and multiplex reactions without compromising data quality.

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Chromatrap® – Targeting the Epigenetic Landscape Alongside Low Abundant Transcription Factor Recruitment
Application Note

This application note demonstrates the sensitivity, selectivity and reproducibility of solid phase Chromatrap®, as an assay for Chromatin IP from low cell numbers.

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Real-time Monitoring of Genetically Encoded Redox Probes in Mammalian Cell Monolayers
Application Note

BMG Labtech

In this application note we show that the PHERAstar FS reader enables roGFP2-based measurements in mammalian cell monolayers grown in 96-well imaging plates.

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On-screen Analysis: Review Captured Cells on-Screen
Application Note

Leica Microsystems

This application note shows the methodology for viewing and evaluating captured cells on screen.

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Improved Library Prep Offers Higher Percentage of On-target Reads and Better Coverage for SureSelectXT2 Target Capture
Application Note

Bioo Scientific

The seamless compatibility between the NEXTflex Pre- and Post- Capture library preparation kit and all reagents required for target capture using Agilent’s SureSelectXT2 bait set is demonstrated.

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Directional qRNA-Seq: Combining the Power of Stranded RNA-Seq with the Quantitative Precision of Molecular Labels
Application Note

Bioo Scientific

Bioo Scientific introduces a novel product for advanced RNA-Seq library preparation that features strand-specific RNA sequencing and correction of PCR amplification bias by molecular indexing: the NEXTflex™ Rapid Directional qRNA-Seq™ Kit.

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Approaching Single-Cell Sequencing by Understanding NGS Library Complexity and Bias
Application Note

Swift Biosciences

This application note describes the sequence coverage performance andp reservation of molecular complexity of next generation sequencing (NGS) libraries generated from human and microbial genomic DNA using the Accel-NGS™ 2S DNA Library Kit for whole-genome sequencing (WGS) on the Illumina® platform.

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