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Application Notes

 
SNP Genotyping of Field Corn Samples with LGC Genomics Chemistry on the Nexar® System
Application Note

Douglas Scientific

A recent study demonstrated that the Nexar® System can be successfully used to produce repeatable and easily scored SNP genotyping results with crude preps from field corn using either KASP or probe-based SNP genotyping assays in miniaturized reaction volumes. This platform also provides significant cost savings in the form of reduced PCR reaction volumes.

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Genotyping of Human Reference DNA Samples with Pharmacologically Important Single Nucleotide Polymorphisms using KASP™ Genotyping Chemistry on the Nexar® System
Application Note

Douglas Scientific

Five pharmacologically important SNPs were analyzed with a miniaturized PCR-based SNP genotyping method using KASP™ genotyping reagents and the Douglas Scientific® Nexar®. The Nexar along with Array Tape® significantly reduces reaction volumes, hands-on effort and over-all cost of SNP genotyping, leading to a reliable and economical alternative to traditional PCR-based SNP genotyping techniques.

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Evaluation of the IntelliQube® for Gene Expression Analysis using a One-Step RT-PCR Workflow
Application Note

Douglas Scientific

A recent study demonstrates the ability of the IntelliQube® and associated Array Tape®, to process RNA samples using a one-step RT-PCR workflow, opening the door to a variety of RNA screening applications including gene expression or viral detection and quantification.

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Rapid point of use DNAble® assay for citrus greening disease (Candidatus Liberibacter spp.) using the Douglas Scientific® AmpliFire®
Application Note

Douglas Scientific

In this experiment, a crude insect preparation and a DNAble assay were used to demonstrate that the AmpliFire instrument accurately detects the presence of HLB-causing bacteria consistent with a real-time PCR system. The AmpliFire offers greater portability than real-time PCR instrumentation, and is a powerful tool for point of use applications such as management of citrus greening disease.

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Microbial Detection and Quantification Using the IntelliQube® from Douglas Scientific®
Application Note

Douglas Scientific

Nucleic Acid Testing (NAT) is a powerful tool for the quantification of microorganisms and are used in many fields. In this study commercially available microbial NAT kits were successfully miniaturized from 20 µL to 1.6 µL reaction volumes using the IntelliQube. The IntelliQube delivers efficient and economical high throughput sample processing for microbial qPCR applications.

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Evaluation of a New Accurate, Automated and Cost-effective Approach for the Analysis of Pharmacologically Important Copy Number Variation
Application Note

Douglas Scientific

The IntelliQube® from Douglas Scientific® is designed to address the need for accurate and reliable results for studies such as CNV determination.

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IntelliQube® Real-Time Quantitative PCR Performance
Application Note

Douglas Scientific

The IntelliQube performs at an industry leading level, facilitating outstanding qPCR results with Array Tape® and the added benefits of inline automation. Liquid handling, thermal cycling, and detection systems within the IntelliQube enables users to achieve efficient and economical high throughput sample processing in Array Tape without compromising important parameters of qPCR performance.

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Reduced-Bias Small RNA Library Preparation with Gel-Free or Low-Input Options
Application Note

Bioo Scientific

Changes in microRNA (miRNA) expression have been shown to be associated with a variety of normal physiological processes, as well as diseases including cancer. Studies have already shown that miRNAs may provide useful markers for the development of disease diagnostic and prognostic assays.

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Characterization of the FlexJet® with Multi-Array Dispense for Reduction of Chemistry Waste Due to Overage
Application Note

Douglas Scientific

This study demonstrates that the Nexar® with the FlexJet multi-array dispense feature can reduce master mix consumed as dead volume by 87.5% compared to Dispense Jet single-array dispense. The capability to perform multi-array dispense with one aspiration also produces repeatable and accurate results and is an ideal solution for genotyping applications.

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Multiplex Genotyping of Human Reference DNA Samples with Pharmacologically Important Single Nucleotide Polymorphisms using the IntelliQube® from Douglas Scientific
Application Note

Douglas Scientific

This study demonstrates the ability of the IntelliQube along with Array Tape®, to successfully achieve two multiplexed SNP genotyping results in a single well and decrease thermal cycling times without compromising data quality. The combination of achieving two results per reaction well and shortened cycling times dramatically increases overall throughput.

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