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Application Notes

 
Automated Illumina TruSeq® Nano DNA Sample Preparation
Application Note

Tecan Group Ltd.

The TruSeq Nano DNA Sample Preparation Kit enables efficient investigation of samples where the amount of DNA available is limited. This low-input method generates excellent quality data from as little at 100 ng of DNA, allowing the study of samples with restricted DNA availability, for example from tumors, and helping to preserve precious sample material for use in future studies.

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Double stranded DNA quantitation using Promega QuantiFluor® Dyes
Application Note

Tecan Group Ltd.

Achieving selective and sensitive quantitation of double stranded DNA (dsDNA) using spectrophotometric methods can be problematic. Recognizing these difficulties, researchers have incorporated fluorescence-based quantitation techniques in their workflows to obtain the required sensitivity and target selectivity.

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Optimized cell culture
Application Note

Tecan Group Ltd.

Culturing of various stable cell lines, including bacterial, yeast, hybridoma, insect and mammalian lines, and stem cells, is an important task in the pharmaceutical industry. The cells need to be maintained and expanded for use in cell-based assays, such as siRNA screening, ADME and high content screening, as well as for clone selection.

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Automating ELISAs on Tecan’s Freedom EVO® using Optimiser™ technology from Siloam Biosciences
Application Note

Tecan Group Ltd.

One of the challenges in life sciences research today is to discover methods for running key assays more quickly, more reliably and using lower volumes of reagents and sample, but still with improved sensitivity. One area where this particularly holds true is for traditional enzyme-linked immunosorbent assays (ELISAs), whose application provides a useful measurement of antigens, including cytokines and a host of other biomarkers.

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Automated Illumina NGS Sample Preparation: TruSeq® Stranded mRNA on the Hamilton Microlab STAR
Application Note

Illumina’s sample preparation kits produce high quality libraries for DNA and RNA sequencing. Hamilton offers the robust Microlab STAR line of robotic liquid handling workstations for automating sample preparation. Together, the automation of the TruSeq Stranded mRNA sample preparation protocol on the STAR gives the user hours of reliable, unattended library preparation.

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Molecular indexing for improved RNA-Seq analysis
Application Note

Bioo Scientific

Most modern methods for nucleic acid analysis require the use of enzyme processing, such as DNA polymerase reactions, in the sample preparation or measurement steps. Although necessary, these enzymatic steps introduce errors in the form of incorrect sequence and misrepresented copy number. NEXTflex™ qRNA-Seq™ Kit from Bioo Scientific adds no additional steps to the work flow, costs no more than a conventional library preparation kit and increases the precision of downstream analysis.

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An Integrated Solution to Simplify Library Preparation and Multiplexing for NimbleGen Sequence Capture
Application Note

Bioo Scientific

The studies described herein demonstrate the capability of Bioo Scientific’s novel NEXTFlex™ DNA Pre-Capture Combo Library construction kits used in conjunction with the NimbleGen Sequence Capture technology to obtain high coverage comparative genomic data from a panel of human HapMap DNA samples.

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BRD4 Bromodomain 1 Inhibitor Screening Using a Homogeneous Proximity Assay
Application Note

BioTek Instruments Inc.

Demonstration of profiling and primary screening of an epigenetic target; a histone acetylating bromodomain. This is a TR-FRET based application highlighting the use of automated instrumentation and a high-throughput screening (HTS) multi-mode microplate reader.

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Purification of Genomic DNA from Saliva Using Oragene® Discover Collection Kits and the Akonni TruTip® Extraction System on the Hamilton Microlab® STAR Liquid Handling Workstation
Application Note

Hamilton

In the following studies, we have demonstrated the high precision of the automated TruTip extraction technology in processing genomic DNA from low-volume saliva samples and its advantage over competitors.

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Rapid, High Throughput Extraction and Purification of Genomic DNA from Whole Blood Using the Akonni TruTip® Extraction System on the Hamilton Microlab® STAR Liquid Handling System
Application Note

Hamilton

This application note demonstrates the high precision of the automated extraction system in processing genomic DNA from low volume whole blood samples and its advantage over competitors.

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