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Application Notes

 
Muliplex SNP Genotyping of Field Corn Crude Samples with Probe-Based Assays using the IntelliQube® from Douglas Scientific®
Application Note

Douglas Scientific

This study evaluated multiplex genotyping on the IntelliQube, a PCR instrument optimized for 1.6 µL reaction volumes in Array Tape®. It demonstrates the feasibility of achieving two SNP genotyping results in a single reaction well. IntelliQube provides cost savings through miniaturized PCR reaction volumes, shorter thermal cycling times, and multiplex reactions without compromising data quality.

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Chromatrap® – Targeting the Epigenetic Landscape Alongside Low Abundant Transcription Factor Recruitment
Application Note

This application note demonstrates the sensitivity, selectivity and reproducibility of solid phase Chromatrap®, as an assay for Chromatin IP from low cell numbers.

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Real-time Monitoring of Genetically Encoded Redox Probes in Mammalian Cell Monolayers
Application Note

BMG Labtech

In this application note we show that the PHERAstar FS reader enables roGFP2-based measurements in mammalian cell monolayers grown in 96-well imaging plates.

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On-screen Analysis: Review Captured Cells on-Screen
Application Note

Leica Microsystems

This application note shows the methodology for viewing and evaluating captured cells on screen.

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Improved Library Prep Offers Higher Percentage of On-target Reads and Better Coverage for SureSelectXT2 Target Capture
Application Note

Bioo Scientific

The seamless compatibility between the NEXTflex Pre- and Post- Capture library preparation kit and all reagents required for target capture using Agilent’s SureSelectXT2 bait set is demonstrated.

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Directional qRNA-Seq: Combining the Power of Stranded RNA-Seq with the Quantitative Precision of Molecular Labels
Application Note

Bioo Scientific

Bioo Scientific introduces a novel product for advanced RNA-Seq library preparation that features strand-specific RNA sequencing and correction of PCR amplification bias by molecular indexing: the NEXTflex™ Rapid Directional qRNA-Seq™ Kit.

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Approaching Single-Cell Sequencing by Understanding NGS Library Complexity and Bias
Application Note

Swift Biosciences

This application note describes the sequence coverage performance andp reservation of molecular complexity of next generation sequencing (NGS) libraries generated from human and microbial genomic DNA using the Accel-NGS™ 2S DNA Library Kit for whole-genome sequencing (WGS) on the Illumina® platform.

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Automated Illumina TruSeq® Nano DNA Sample Preparation
Application Note

Tecan Group Ltd.

The TruSeq Nano DNA Sample Preparation Kit enables efficient investigation of samples where the amount of DNA available is limited. This low-input method generates excellent quality data from as little at 100 ng of DNA, allowing the study of samples with restricted DNA availability, for example from tumors, and helping to preserve precious sample material for use in future studies.

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Double stranded DNA quantitation using Promega QuantiFluor® Dyes
Application Note

Tecan Group Ltd.

Achieving selective and sensitive quantitation of double stranded DNA (dsDNA) using spectrophotometric methods can be problematic. Recognizing these difficulties, researchers have incorporated fluorescence-based quantitation techniques in their workflows to obtain the required sensitivity and target selectivity.

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Optimized cell culture
Application Note

Tecan Group Ltd.

Culturing of various stable cell lines, including bacterial, yeast, hybridoma, insect and mammalian lines, and stem cells, is an important task in the pharmaceutical industry. The cells need to be maintained and expanded for use in cell-based assays, such as siRNA screening, ADME and high content screening, as well as for clone selection.

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