Automated Illumina NGS Sample Preparation: TruSeq® Stranded mRNA on the Hamilton Microlab STAR
Application Note Jan 28, 2014
The Illumina® TruSeq Stranded mRNA Sample Preparation kit provides reagents and a workflow for converting the mRNA in total RNA into a library of template molecules of known strand origin, which is then suitable for cluster generation and DNA sequencing. The kit has been verified for use on the Microlab STAR liquid handling workstation and the automation protocol has been Illumina Qualified*. Implementation of both the low throughput (LT) and high throughput (HT) sample preparation kits on the STAR have resulted in high yield and high quality libraries.