Automated Illumina TruSeq® Nano DNA Sample Preparation
Application Note Oct 02, 2014
Tecan Group Ltd.
The automated workflow includes simple bead-based size selection, avoiding the sample losses typically associated with gel-based selection, and can be completed in less than a day with minimal hands-on time. In addition, the protocol enables a variety of read lengths to be selected, meeting the everincreasing read lengths of Illumina sequencing instruments. This application note describes an automated-protocol for parallel processing of up to 48 samples using TruSeq Nano DNA Sample Preparation Kits on the Freedom EVO NGS workstation. The user-friendly TouchTools™ interface guides the operator through automation set-up to deliver highly reproducible, sequencing-ready DNA libraries for single read, paired-end and indexed sequencing with minimal user intervention.