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CIRCLE-Seq for CRISPR/Cas9 Off-Target Screening

CRISPR/Cas9 gene editing can result in off-target cleavage events, impacting the validity of your final results. Cleavage of unintended targets can cause impaired cell function, cell death or misleading conclusions. CIRCLE-seq is a sensitive in vitro assay that was developed to overcome the challenges associated with off-target screening.


Download this app note to discover a method that helps you combat these challenges by:

  • Reducing error rate by up to 100 times
  • Ensuring uniform sequence coverage
  • Detecting more off-target events than other methods used to screen for off-target Cas9 cleavage
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