CIRCLE-Seq for CRISPR/Cas9 Off-Target Screening
CRISPR/Cas9 gene editing can result in off-target cleavage events, impacting the validity of your final results. Cleavage of unintended targets can cause impaired cell function, cell death or misleading conclusions. CIRCLE-seq is a sensitive in vitro assay that was developed to overcome the challenges associated with off-target screening.
Download this app note to discover a method that helps you combat these challenges by:
- Reducing error rate by up to 100 times
- Ensuring uniform sequence coverage
- Detecting more off-target events than other methods used to screen for off-target Cas9 cleavage